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portada Purinergic Signaling: Methods and Protocols
Type
Physical Book
Publisher
Author
Language
English
Pages
360
Format
Paperback
ISBN13
9781493997190
Edition No.
1
Categories

Purinergic Signaling: Methods and Protocols

Pelegrín, Pablo (Author) · Humana · Paperback

Purinergic Signaling: Methods and Protocols - Pelegrín, Pablo

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Synopsis "Purinergic Signaling: Methods and Protocols"

This volume aims to cover all major methodological aspects of research into purinergic signaling and to provide a foundation for studying them at molecular, biochemical, pharmacological, and physiological levels. Chapters guide readers through current knock-out and knock-in mouse models, in silico modeling, knock down purinoceptor expression, bioluminiscence resonance energy transfer, enzyme-based biosensors, recording P2X receptor electrophysiology, controlling P2X receptors by optogenetics, inflammasome activation, leukocyte migration, and cell adhesion. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Purinergic Signaling: Methods and Protocols will provide asound basis for molecular, cellular, and physiological research into purinergic signaling in health and disease and will spark interest in this fascinating signaling process among researchers in many different and unrelated disciplines.

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