Single-Cell Protein Analysis: Methods and Protocols (Methods in Molecular Biology)
Ooi Aik T.
Synopsis "Single-Cell Protein Analysis: Methods and Protocols (Methods in Molecular Biology)"
1. Immunochemistry Analysis Using Chromogenic Substrates on Tissue Sections Preethi Vijayaraj 2. Indirect Immunofluorescence of Tissue Sections Cody J. Aros 3. Flow Cytometry for Beginners: Hints and Tips for Approaching the Very First Single-Cell Technique Valentina Proserpio, Laura Conti, and Salvatore Oliviero 4. High-Dimensional Immunophenotyping with 37-Color Panel Using Full-Spectrum Cytometry Marco A. Fernandez, Hammad Alzayat, Maria C. Jaimes, Yacine Kharraz, Gerard Requena, and Pedro Mendez 5. Detection of Cytokine Secreting Cells by Enzyme-Linked Immunospot (ELISpot) Bernt Axelsson 6. Detection and Enumeration of Cytokine Secreting Cells by FluoroSpot Bernt Axelsson 7. Single Cell Protein Profiling by Microdroplet Barcoding and Next Generation Sequencing Samuel C. Kim, John R. Haliburton, Zev J. Gartner, and Adam R. Abate 8. Single Cell Proteomics Using Multiplexed Isobaric Labeling for Mass Spectrometric Analysis Ákos Végvári, Jimmy E. Rodrigues, and Roman A. Zubarev 9. Customizing Maxpar Direct Immune Profiling Assay with Additional Surface Marker and Intracellular Cytokine Staining Workflows for Expanded Mass Cytometry Panels Carlene Petes, Stephen K.H. Li, Shariq Mujib, Michelle M. Poulin, Noah Saederup, Andrew A. Quong, and Christina Loh 10. High-Dimensional Tissue Profiling by Multiplexed Ion Beam Imaging Ofer Elhanani, Leeat Keren, and Michael Angelo 11. Ultra-Sensitive Quantification of Protein and mRNA in Single Mammalian Cells with Digital PLA Jing Lin and Savaş Tay 12. High-Throughput Multimodal Single-Cell Targeted DNA and Surface Protein Analysis Using the Mission Bio Tapestri Platform David W. Ruff, Dalia Dhingra, Kathryn Thompson, Jacqueline A. Marin, and Aik T. Ooi 13. Simultaneous Analysis of Single-Cell Transcriptomes and Cell Surface Protein Expression of Human Hematopoietic Stem Cells and Progenitors Using the 10x Genomics Platform Nicole Mende, Elisa Laurenti, Berthold Gottgens, and Nicola K. Wilson 14. Generation of Centered Log-Ratio Normalized Antibody-Derived Tag Counts from Large Single-Cell Sequencing Datasets Benjamin Lacar 15. Simultaneous Quantification of Single-Cell Proteomes and Transcriptomes in Integrated Fluidic Circuits Mandi Wong, Carol Kosman, Liane Takahashi, and Naveen Ramalingam 16. Combined Measurement of RNA and Protein Expression on a Single-Cell Level
