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portada Crispr-Cas Methods (Springer Protocols Handbooks)
Type
Physical Book
Publisher
Author
Language
English
Pages
292
Format
Hardcover
ISBN13
9781071606155
Edition No.
1

Crispr-Cas Methods (Springer Protocols Handbooks)

Islam M. Tofazzal|Bhowmik Pankaj K.|Molla Kutubuddin A. (Author) · Humana · Hardcover

Crispr-Cas Methods (Springer Protocols Handbooks) - Islam M. Tofazzal|Bhowmik Pankaj K.|Molla Kutubuddin A.

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Synopsis "Crispr-Cas Methods (Springer Protocols Handbooks)"

This volume details the fundamentals of the CRISPR-Cas system, and its protocols illustrate advances in CRISPR-Cas techniques for efficient genome editing. Introductory chapters provide a wide horizon of CRISPR/Cas-based methods and applications. Additional chapters guide readers through HDR-mediated editing, sgRNA design, the step-by-step procedure of multiplex adenine base editing experiments in rice, generating mutants for rice, wheat, Brachypodium, Barley, Flax, and Phytophthora, visual screening of mutants, gene deletion (knock-out), tagging (knock-in) in mammalian cells, the cloning-free (DNA-free) technique, cell-penetrating peptides, generating a genome-edited banana, and nuclear genome editing of Chlamydomonas employing CRISPR-Cpf1 combined with a single-stranded DNA (ssODN) repair template. Authoritative and cutting-edge, CRISPR-Cas Methods aims to assist researchers who are new to the field and are aiming to learn how best to adopt this technology for a particular organism.

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