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portada Directed Evolution: Methods and Protocols (Methods in Molecular Biology, 2461)
Type
Physical Book
Publisher
Author
Language
English
Pages
289
Format
Hardcover
ISBN13
9781071621516
Edition No.
1

Directed Evolution: Methods and Protocols (Methods in Molecular Biology, 2461)

Currin Andrew,Swainston Neil (Author) · Humana · Hardcover

Directed Evolution: Methods and Protocols (Methods in Molecular Biology, 2461) - Currin Andrew,Swainston Neil

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Synopsis "Directed Evolution: Methods and Protocols (Methods in Molecular Biology, 2461)"

This volume explores the latest techniques used by researchers to study directed evolution (DE) at each stage of the Design-Build-Test-Learn cycle. Chapters in this book cover topics such as designing overlap extension PCR primers for protein mutagenesis; antha-guided automation of Darwin assembly for the construction of bespoke gene libraries; rapid cloning of random mutagenesis libraries using PTO-Quickstep; and DE of glycosyltransferases by a single-cell screening method. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Directed Evolution: Methods and Protocols is a valuable resource for scientists and researchers who are interested in learning more about this field and incorporating these studies into new experimental workflows.

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