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portada DNA Vaccines: Methods and Protocols
Type
Physical Book
Publisher
Author
Language
English
Pages
529
Format
Paperback
Dimensions
23.4x15.6x2.8 cm
Weight
0.76 kg.
ISBN13
9781489941824
Categories

DNA Vaccines: Methods and Protocols

Lowrie, Douglas B. ; Whalen, Robert (Author) · Humana · Paperback

DNA Vaccines: Methods and Protocols - Lowrie, Douglas B. ; Whalen, Robert

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Synopsis "DNA Vaccines: Methods and Protocols"

The field of DNA vaccines has undergone explosive growth in the last few years. As usual, some historical precursors of this approach can be d- cerned in the scientific literature of the last decades. However, the present state of affairs appears to date from observations made discreetly in 1988 by Wolff, Malone, Felgner, and colleagues, which were described in a 1989 patent and published in 1990. Quite surprisingly, they showed that genes carried by pure plasmid DNA and injected in a saline solution, hence the epithet "naked DNA," could be taken up and expressed by skeletal muscle cells with a low but reproducible frequency. Such a simple methodology was sure to spawn many applications. In a separate and important line of experimentation, Tang, De Vit, and Johnston announced in 1992 that it was indeed possible to obtain humoral immune responses against proteins encoded by DNA delivered to the skin by a biolistic device, which has colloquially become known as the "gene gun. " The year 1993 saw the publication of further improvements in the me- ods of naked DNA delivery and, above all, the first demonstrations by several groups of the induction of humoral and cytotoxic immune responses to viral antigens expressed from injected plasmid DNA. In some cases, protection against challenge with the pathogen was obtained. The latter result was - questionably the touchstone of a method of vaccination worthy of the name.

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