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portada mobile genetic elements
Type
Physical Book
Illustrated by
Publisher
Author
Year
2010
Language
English
Pages
292
Format
Paperback
Dimensions
22.9x15.2x1.7 cm
Weight
0.45 kg.
ISBN
161737301x
ISBN13
9781617373015
Categories

mobile genetic elements

Wolfgang J. Miller (Illustrated by) · Pierre Capy (Illustrated by) · Humana · Paperback

mobile genetic elements - Miller, Wolfgang J. ; Capy, Pierre

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Synopsis "mobile genetic elements"

Mobile Genetic Elements: Protocols and Genomic Applications brings together a wide array of transposon-based protocols and stategies for studying genome structure, function, and evolution into a highly practical, single-source volume. Such transposable element (TE)-derived techniques have been applied succe- fully for a variety of purposes ranging from mutagenesis, gene silencing, transgenesis, and their use as polymorphic marker systems. To our knowledge no such synthesis has been presented before. Chapters 2-4 provide a series of DNA hybridization techniques for anal- ing the distribution and dynamics of mobile DNAs at the hosts' genomic level. With the current revolution in genomics and the availability of complete genome sequences, computational analyses provide an extremely powerful tool for i- lating and investigating TEs at the in silico level (Chapter 5). For the analyses of transpositional mechanisms at the biochemical level Chapter 6 provides a detailed protocol for LTR retrotransposons in heterologous host systems. Ch- ters 7-10 are focused on TE-based mutagenesis protocols for studying gene functions in a broad range of organisms. Based on their ubiquitous nature and their activity in creating genomic diversity by integrating novel DNA segments into genomes, TEs provide highly informative sets of polymorphic markers (Chapters 11-13). Finally, the last two chapters are dedicated to their technical applications during transgenesis in arthropods and vertebrates.

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