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portada mrna processing and metabolism
mrna processing and metabolismmrna processing and metabolism
Type
Physical Book
Publisher
Language
English
Pages
288
ISBN
1588292258
ISBN13
9781588292254
Categories

mrna processing and metabolism

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Synopsis "mrna processing and metabolism"

a collection of readily reproducible techniques for the study of mrna processing and metabolism. these cutting-edge techniques range from cotranscriptional processing events that occur while the mna is engaged with elongating rna polymerase ii, to in vivo and in vitro splicing and its biochemical analysis, and alternative splicing. additional methods cover mrna export, the recovery and analysis of mrnp complexes, cytoplasmic translation, mrna degradation in vivo and in vitro, and the controversial concept of nuclear translation. each proven protocol is described in step-by-step detail and contains a background introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding know pitfalls.

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