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portada pcr cloning protocols
Type
Physical Book
Illustrated by
Publisher
Author
Year
2010
Language
English
Pages
439
Format
Paperback
Dimensions
22.9x15.2x2.6 cm
Weight
0.66 kg.
ISBN
1617372811
ISBN13
9781617372810
Edition No.
0002
Categories

pcr cloning protocols

Bing-Yuan Chen (Illustrated by) · Harry W. Janes (Illustrated by) · Humana · Paperback

pcr cloning protocols - Chen, Bing-Yuan ; Janes, Harry W.

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Synopsis "pcr cloning protocols"

PCR Cloning Protocols, Second Edition, updates and expands Bruce White's best-selling PCR Cloning Protocols (1997) with the newest procedures for DNA cloning and mutagenesis. Here the researcher will find readily reproducible methods for all the major aspects of PCR use, including PCR optimization, computer programs for PCR primer design and analysis, and novel variations for cloning genes of special characteristics or origin, with emphasis on long distance PCR and GC-rich template amplification. Also included are both conventional and novel enzyme-free and restriction site-free procedures to clone PCR products into a range of vectors, as well as state-of-the-art protocols to facilitate DNA mutagenesis and recombination, and to clone the challenging uncharacterized DNA flanking a known DNA fragment.

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