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portada Phosphoinositides: Methods and Protocols (Methods in Molecular Biology)
Type
Physical Book
Publisher
Author
Language
English
Pages
252
Format
Paperback
ISBN13
9781071611449
Edition No.
1

Phosphoinositides: Methods and Protocols (Methods in Molecular Biology)

Botelho Roberto J. (Author) · Humana · Paperback

Phosphoinositides: Methods and Protocols (Methods in Molecular Biology) - Botelho Roberto J.

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Synopsis "Phosphoinositides: Methods and Protocols (Methods in Molecular Biology)"

This detailed book examines experimental approaches used to investigate the regulation and function of phosphoinositides (PtdInsP), rare eukaryotic phospholipids with a broad role in biological processes such as signal transduction, cell migration and adhesion, cell growth, subcellular organization, and membrane trafficking. The combination of complementary biochemical, mass spectrometry, and imaging methods are instrumental for the detection and quantification of PtdInsP species, as well as induced dimerization methods, affinity precipitation or co-sedimentation with liposomes, protein insertion within lipid bilayers, and enzymatic assays, or through emerging methods like native mass spectrometry and microfluidics, all of which are touched upon in this volume. Written in the highly successful Methods in Molecular Biology format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Phosphoinositides: Methods and Protocols provides detailed methodology for both specialist and novice researchers on a variety of complementary methods that have been instrumental in dissecting the regulation, dynamics, and function of PtdInsPs.

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