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portada single cell diagnostics: methods and protocols
Type
Physical Book
Illustrated by
Publisher
Author
Year
2010
Language
English
Pages
182
Format
Paperback
Dimensions
22.9x15.2x1.1 cm
Weight
0.29 kg.
ISBN
161737654x
ISBN13
9781617376542
Categories

single cell diagnostics: methods and protocols

Alan R. Thornhill (Illustrated by) · Humana · Paperback

single cell diagnostics: methods and protocols - Thornhill, Alan R.

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Synopsis "single cell diagnostics: methods and protocols"

Single Cell Diagnostics: Methods and Protocols applies modernmolecular diagnostic techniques to the analysis of single cells, smallnumbers of cells, or cell extracts. Emphasis is placed on non-invasiveanalysis of single cell metabolites and the direct analysis of RNA andDNA from single cells, with a focus on polymerase chain reaction andfluorescence in situ hybridization. The authors, all leadingscientists in the field, provide current and innovative methods forextracting useful, accurate, and reliable data from single-cellsamples. The stepwise, technically precise protocols presented in thisvolume thoroughly describe single-cell assays, from material collectionand isolation of cells to data interpretation. Cell-types analyzedinclude embryonic blastomeres, fibroblasts, and lymphocytes. Among thetechniques described are real-time quantitative PCR, isothermal wholegenome amplification, comparative genomic hybridization, real-timegenetic expression analysis, and the production of RNA and cDNAlibraries. This handbook will be essential for researchers andclinicians, and practitioners providing care for couples seekingtreatment for infertility or preimplantation genetic diagnosis.

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