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portada Thermogenic Fat: Methods and Protocols
Type
Physical Book
Publisher
Author
Language
English
Pages
253
Format
Hardcover
Dimensions
25.4x17.8x1.6 cm
Weight
0.68 kg.
ISBN13
9781071631669
Categories

Thermogenic Fat: Methods and Protocols

Lodhi, Irfan J. (Author) · Humana · Hardcover

Thermogenic Fat: Methods and Protocols - Lodhi, Irfan J.

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Synopsis "Thermogenic Fat: Methods and Protocols"

This volume presents a broad collection of state-of-the-art methods to study the biology of thermogenic fat using in vitro cell culture and animal models. Chapters guide the readers through protocols on differentiation of human pluripotent stem cells and murine adipocyte precursors; methods for measuring mitochondrial respiration, heat generation, brown fat activation, and effects on energy metabolism in mice; and techniques for AAV-mediated gene delivery, transplantation of adipose tissue, isolation of adipose tissue immune cells and extracellular vesicles, and mass spectrometry-based profiling of brown fat lipids. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Thermogenic Fat: Methods and Protocols aims to be comprehensive guide for researchers in the field.

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