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portada Bacterial Gene Expression Technologies
Type
Physical Book
Publisher
Author
Year
2017
Language
English
Pages
124
Format
Paperback
ISBN13
9786202004787

Bacterial Gene Expression Technologies

Mahmoud Zaky; Merna Abou-Selima (Author) · Lap Lambert Academic Publishing · Paperback

Bacterial Gene Expression Technologies - Mahmoud Zaky; Merna Abou-Selima

New Book Imported to Taiwan
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NT$ 1,908

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Synopsis "Bacterial Gene Expression Technologies "

Random integration of the IVET vector into the pathogen chromosome is performed by insertion–duplication mutagenesis to create a pool of recombinant pathogens (this means that the gene in which the vector has inserted by homologous recombination is not disrupted). Recombinants can be selected using antibiotic resistance, as an additional marker is also on the integrated IVET construct. Pooled clones are then inoculated into the mouse. The two main types of IVET promoter trap strategies are (a) the complementation of auxotrophic mutation and (b) the expression of antibiotic resistance. Only those bacteria that contain the selective marker fused to a gene that is transcriptionally active in the host are able to survive. After a suitable infection period, bacteria that express the marker are isolated from lung, liver, spleen, and mesenteric tissue.

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