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portada Baculovirus and Insect Cell Expression Protocols
Type
Physical Book
Publisher
Author
Language
English
Pages
508
Format
Paperback
Dimensions
25.4 x 17.8 x 2.7 cm
Weight
0.89 kg.
ISBN13
9781493979783
Edition No.
0003
Categories

Baculovirus and Insect Cell Expression Protocols

Murhammer, David W. (Author) · Humana · Paperback

Baculovirus and Insect Cell Expression Protocols - Murhammer, David W.

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Synopsis "Baculovirus and Insect Cell Expression Protocols"

The third edition of this volume expands upon the previous two editions with new and up-to-date methods and protocols. Chapters include step-by-step procedures involved in quantifying cell growth, baculovirus infection and cell metabolism, methods to isolate new cell lines and develop your own serum-free medium, and routine maintenance and storage of insect cell lines and baculoviruses, small- and large-scale recombinant protein production with the BEVS in both insect and mammalian cell culture and in insect larvae, production and characterization of baculoviruses, green fluorescent protein, tubular reactors and RNAi, and baculovirus/insect cell system to study apoptosis and generating envelop-modified baculovirus for gene delivery into mammalian cells. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Baculovirus and Insect Cell Expression Protocols, Third Edition aims to not only aid the user in successfully completing the tasks described, but also stimulate the development of improved techniques and new applications of baculoviruses and insect cell culture.

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