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portada Mass Spectrometry-Based Proteomics
Type
Physical Book
Collection
Methods in Molecular Biology
Year
2024
Pages
336
Format
Paperback
Dimensions
25.4x17.8 cm
ISBN13
9781071634592

Mass Spectrometry-Based Proteomics

Kris Gevaert (Author) · Springer-Verlag New York Inc. · Paperback

Mass Spectrometry-Based Proteomics - Kris Gevaert

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Synopsis "Mass Spectrometry-Based Proteomics"

This detailed volume explores contemporary techniques in mass spectrometry-based proteomics. After covering overall proteome coverage and the cellular surfaceome, the book delves into proximity-induced biotinylation, abduction of protein complexes in viral-like particles, and thermal proteome profiling, as well as protocols for identifying protein N-terminal acetylation, protein processing by proteases, protein N-glycosylation, and protein phosphorylation. The book also collects chapters on automated preparation of clinical samples, the analysis of formalin-fixed paraffin-embedded samples, protocols for the isolation of extracellular vesicles and for the monitoring of selected protein modifications in clinical samples, and, finally, structural proteomics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mass Spectrometry-Based Proteomics serves as an ideal guide to its subject for both novices in the field of proteomics as well as specialists.

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