Melanoma: Methods and Protocols - Kristian M. Hargadon

This fully updated edition brings together leading melanoma researchers from across the world and highlights cutting-edge protocols and experimental systems currently being used to investigate questions surrounding this disease. Beginning with methodology for genetically engineering melanoma cells, the book continues by covering 2D and 3D cell culture-based approaches for studying melanoma biology, the isolation and study of circulating melanoma cells and melanoma-derived extracellular vesicles, melanoma imaging techniques, computational omics-based approaches, as well as methods related to the development and testing of melanoma diagnostics and therapeutics. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips for troubleshooting and avoiding known pitfalls. 

Authoritative and comprehensive, Melanoma: Methods and Protocols, Second Edition serves basic research scientists and clinicians who bring questions from the clinic into the lab in order to translate observations in the laboratory into improved patient care for this highly malignant form of cancer.