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portada The Escrt Complexes: Methods and Protocols (Methods in Molecular Biology)
Type
Physical Book
Publisher
Year
2019
Language
English
Pages
322
Format
Hardcover
ISBN13
9781493994915
Edition No.
1
Categories

The Escrt Complexes: Methods and Protocols (Methods in Molecular Biology)

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Synopsis "The Escrt Complexes: Methods and Protocols (Methods in Molecular Biology) "

This detailed collection gathers both established and recent technical procedures to study the Endosomal Sorting Complex Required for Transport (ESCRT) complexes in a wide range of biological systems: Archaea, A. thaliana, U. maydis, S. cerevisiae, S. pombe, C. elegans, D. melanogaster, and mammalian cells. Opening with a section on imaging techniques, the book continues with chapters covering biochemical approaches presenting strategies for production and characterization of recombinant ESCRT proteins, or of specific ESCRT protein domains, as well as genetic and proteomic experimental approaches. Written for the highly successful Methods in Molecular Biology series, chapters include introduction to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The ESCRT Complexes: Methods and Protocols serves as a compact guide for researchers interested in establishing an integrated approach to investigate the ESCRT machinery functions in cell biology.

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